Evaluation of Antibiotics Ampicillin and Ciprofloxacin and Biosurfactant Rhamnolipid Effect on Hydrophobicity and Electron Donors and Recipients in Urinary and Fecal Escherichia Coli
Mahdi Khangholi,
Shaghayegh Anvari,
Ezzatollah Ghaemi,
Ailar Jamalli
Issue:
Volume 6, Issue 3, June 2018
Pages:
39-46
Received:
27 July 2018
Accepted:
4 October 2018
Published:
6 November 2018
Abstract: Adhesion is the first and most important stage in pathogenesis after bacteria enters to the body. Attachment of bacteria in medicine, industry, agriculture, waste decomposition, shipbuilding, etc. is important. Hydrophobicity and electron donor- electron acceptor characteristic are more important factors in bacterial adhesion. This study tries examining effect of biosurfactant rhamnolipid and two antibiotics ampicillin and ciprofloxacin on E. coli cell surface hydrophobicity and electron donor- electron acceptor characteristic by MATS method in terms of sample type and antibiotic resistance. Isolated bacteria from urine samples has a more antibiotic resistance to ampicillin. The results indicate that rhamnolipid makes increase in hydrophobicity and electron donor characteristic and in opposite ciprofloxacin makes increase electron acceptor and in opposite and decrease hydrophobicity. Also, hydrophobicity and electron donor- electron acceptor characteristic were different in sensitive and resistant to antibiotics strains. This study results showed since the hydrophobicity and electronic exchange are important factors involved in attachment of bacteria to inanimate surfaces and inner surfaces of the body, we can inhibit bacterial binding to it and help to reduce the incidence of antibiotic resistance by change these surfaces. The effect of antibiotics and rhamnolipid on some effective characteristic in adhesion cannot be ignored, despite the difference in their impact.
Abstract: Adhesion is the first and most important stage in pathogenesis after bacteria enters to the body. Attachment of bacteria in medicine, industry, agriculture, waste decomposition, shipbuilding, etc. is important. Hydrophobicity and electron donor- electron acceptor characteristic are more important factors in bacterial adhesion. This study tries exam...
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Protective Effect of Valsartan on Podocyte Injury in Rats with Diabetic Nephropathy
Qingfen Wang,
Rui Li,
Weiwei Li,
Lei Wang
Issue:
Volume 6, Issue 3, June 2018
Pages:
47-51
Received:
5 December 2018
Published:
6 December 2018
Abstract: To investigate the mechanism of valsartan protecting podocytes and inhibiting renal injury in diabetic rats. The rat model of diabetic nephropathy was induced by combination of valsartan and high-sugar and high-fat diet. The urinary protein content, renal index, inflammatory and antioxidant indexes in the kidney, renal pathological changes and podocyte holes were investigated. Membrane WT1 and P-Cadherin protein expression levels. Compared with the model group, the 24h urine protein content of valsartan was significantly decreased (P<0.05). Valsartan significantly inhibited the body weight of the model group (P<0.01), and significantly inhibited The increase of renal index (P<0.05); the high and middle doses of valsartan could significantly reduce the levels of IL-β, TNF-α and IL-6 in rat kidney (P<0.05-0.01). The valsartan high and middle dose groups significantly reduced MDA content in rat kidney (P<0.05), and significantly increased SOD activity (P<0.05). HE and PAS staining showed that valsartan was used in model group rats. The pathological changes were alleviated, and the glomerular morphology returned to normal. The protein expression of WT1 and P-Cadherin in the kidney of DN rats by Western blot showed that P- in the kidney tissue of valsartan rats. The expression levels of Cadherin and WT1 protein were significantly increased (P<0.05). Valsartan can regulate the expression of podocyte membrane proteins WT1 and P-Cadherin to protect podocytes, and then repair renal function and anti-diabetic nephropathy.
Abstract: To investigate the mechanism of valsartan protecting podocytes and inhibiting renal injury in diabetic rats. The rat model of diabetic nephropathy was induced by combination of valsartan and high-sugar and high-fat diet. The urinary protein content, renal index, inflammatory and antioxidant indexes in the kidney, renal pathological changes and podo...
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