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Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent
Amechi Sampson Nwankwegu,
Emmanuel Michael Ikpe,
Edna Ifeoma Chukwura,
Richard Chidiebere Irondi,
Emmanuel Ifeanyi Obika
Issue:
Volume 4, Issue 3, June 2016
Pages:
71-75
Received:
18 May 2016
Accepted:
27 May 2016
Published:
14 June 2016
Abstract: Bioremediation of crude oil is quite unique, complex and relatively a slow process. They study area for this research work was a cottage industry located in Orumba North local government area of Anambra state, Nigeria. Evaluating the degradation potential of bacterial isolates from palm oil mill effluent on crude oil was conducted using standard methods, which includes screening, inoculums development and degradation studies to assess the most promising hydrocarbon degraders. Pooled samples collected from different points on the palm oil mill effluent disposal channels were inoculated mineral salt medium containing crude oil to assess degradation abilities of different bacterial species. Data indicated that these bacterial isolates were capable of thriving in the mineral salt medium between the pH range of 6.0-7.0 and bacterial proliferation were quantified by assessing the total viable count which ranged between 2 x 105-9 x 105 cfu/ml based on individual abilities to utilize crude oil as a sole carbon source. Cultural, morphological and biochemical characterizations were conducted on these isolates and Bergey’s manual of determinative bacteriology was used to suggest possible isolates, while molecular identification was done Centre for Agriculture and biosciences International (CABI), UK using partial 16S rDNA sequencing analysis and FASTA algorithm with prokaryote database from EBI. Escherichia fergosonii, Klebsiella variicola and Microccocus luteus were identified. Gas chromatographic analysis was applied to quantify the extent of degradation of crude oil by the isolates. All the isolated bacterial species displayed varying degree of crude hydrocarbon biodegradation.
Abstract: Bioremediation of crude oil is quite unique, complex and relatively a slow process. They study area for this research work was a cottage industry located in Orumba North local government area of Anambra state, Nigeria. Evaluating the degradation potential of bacterial isolates from palm oil mill effluent on crude oil was conducted using standard me...
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In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants
Sr. Sagaya Mary B.,
Divakar K. M.
Issue:
Volume 4, Issue 3, June 2016
Pages:
76-81
Received:
29 April 2016
Accepted:
20 May 2016
Published:
17 June 2016
Abstract: Dendrobium jerdonianum Wight is an Epiphytic tufted orchid at higher elevations. The maximum percentage establishment of Flower stalk, leaf, node and internode explants was observed on MS, VW and KC Medium. However, MS media fortified with activated charcoal exhibited maximum multiplication rate for in vitro rooting. MS, VW and KC media supplemented with various concentrations of auxins and cytokinins were used in flower stalk, leaf, nodes and internodes for plantlet formation. In the evaluation of the media MS basal medium fortified with 0.5mg 2, 4, D-dichloro phenoxy acetic acid +5mg BAP + 50mlsuitable for flower stalk culture. KC medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid was found to be suitable for Leaf and internodes Culture. VW medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 3mg BAP + 50ml CM, was found to be suitable for nodal segments. The entire above medium used with different composition gives highest percentage of 80-95 percent results for plantlet formation. In vitro rooting was successful with MS medium supplemented with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 5mg IAA + 50ml CM and 500mg of activated charcoal. 90 days old in vitro plantlets were hardened and transferred to green house after ex vitro rooting technique. Significance of the present work is discussed here.
Abstract: Dendrobium jerdonianum Wight is an Epiphytic tufted orchid at higher elevations. The maximum percentage establishment of Flower stalk, leaf, node and internode explants was observed on MS, VW and KC Medium. However, MS media fortified with activated charcoal exhibited maximum multiplication rate for in vitro rooting. MS, VW and KC media supplement...
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Characterization of Myostatin Gene in Nile Tilapia (Oreochromis niloticus), the Possible Association of BsmI-exon 2 Polymorphism with Its Growth
Nasema A. Elkatatny,
Zizy I. Elbialy,
Abeer F. El-Nahas,
Shawky Mahmoud
Issue:
Volume 4, Issue 3, June 2016
Pages:
82-86
Received:
30 April 2016
Accepted:
1 June 2016
Published:
17 June 2016
Abstract: Myostatin (MSTN) gene is a negative regulator of skeletal muscle. In this study, we try to evaluate MSTN as a candidate gene for a marker assisted selection in Nile tilapia growth trait. Molecular characterization of MSTN gene was done using seven designed primers. We demonstrated that, O. niloticus MSTN sequence and its promoter is as for all known vertebrates. Novel SNPs were identified in coding and non-coding regions compared with MSTN gene of O. niloticus x O. aureus hybrid, three non-synonymous SNPs were found at MSTN coding region; two at exon one 369 C>A, 831 T>A, and one at exon 3 2637 G>A, altering Thr 38-Pro, Glu 121-Val and Tyr 375-Cys respectively. Phylogenetic analysis revealed high similarity (99.2) with MSTN gene of the hybrid with O. aurous. BsmI induced cutting pattern at MSTN-exon 2 (607-bp). Among two hundred monosex male fish, two different genotypes were reported; AB genotype (607-bp, 507-bp and 100-bp) and BB genotype (507-bp and 100-bp fragments), were produced. Most large-size fish are included in AB genotype with 0.8 frequency and significantly increased body weight compared with small size fish, which are mostly included in BB genotype with 0.9 frequencies. Novel BsmI-exon 2 polymorphism of MSTN gene can be used as a marker assisted selection for large body weight in heterozygous Nile tilapia fish.
Abstract: Myostatin (MSTN) gene is a negative regulator of skeletal muscle. In this study, we try to evaluate MSTN as a candidate gene for a marker assisted selection in Nile tilapia growth trait. Molecular characterization of MSTN gene was done using seven designed primers. We demonstrated that, O. niloticus MSTN sequence and its promoter is as for all know...
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Effect of Nigella sativa (Black Cumin Seed) to Enhance the Immunity of Common Carp (Cyprinus carpio) Against Pseudomonas fluorescens
Shoumo Khondoker,
Md. Mer Mosharraf Hossain,
Md. Hasan-Uj-Jaman,
Md. Eftakher Alam,
Md. Farid Uz Zaman,
Nazia Tabassum
Issue:
Volume 4, Issue 3, June 2016
Pages:
87-92
Received:
12 May 2016
Accepted:
23 May 2016
Published:
17 June 2016
Abstract: The effect of Nigella sativa (black cumin) seed extracts supplemented diets at 2%, 4% and 6% doses with common diet as control (0%) fed to common carp, Cyprinus carpio against Pseudomonas fluorescens was investigated critically on weeks 1, 2 and 4 as immunomodulator. Immunological factors including bactericidal activity and phagocytic action were examined along with the disease resistance. The outcomes of the research recommend 4% dose of N. sativa oil enriched diet as an effective immune response and disease resistance agent for C. carpio against P. fluorescens although further investigation will facilitate to optimize the precise quantity comparing with the weight of fish body.
Abstract: The effect of Nigella sativa (black cumin) seed extracts supplemented diets at 2%, 4% and 6% doses with common diet as control (0%) fed to common carp, Cyprinus carpio against Pseudomonas fluorescens was investigated critically on weeks 1, 2 and 4 as immunomodulator. Immunological factors including bactericidal activity and phagocytic action were e...
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