Impact of Biofield Energy Treated (The Trivedi Effect®) Herbomineral Formulation on the Immune Biomarkers and Blood Related Parameters of Female Sprague Dawley Rats
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Aileen Carol Lee,
Aksana Hancharuk,
Carola Marina Sand,
Debra Jane Schnitzer,
Rudina Thanasi,
Eileen Mary Meagher,
Faith Ann Pyka,
Gary Richard Gerber,
Johanna Catharina Stromsnas,
Judith Marian Shapiro,
Laura Nelson Streicher,
Lorraine Marie Hachfeld,
Matthew Charles Hornung,
Patricia M. Rowe,
Sally Jean Henderson,
Sheila Maureen Benson,
Shirley Theresa Holmlund,
Stephen Phillip Salters,
Mayank Gangwar,
Snehasis Jana
Issue:
Volume 5, Issue 6, December 2017
Pages:
150-159
Received:
16 October 2017
Accepted:
30 October 2017
Published:
11 December 2017
Abstract: The aim of the present study was to evaluate the immunomodulatory potential of Biofield Energy Treatment (The Trivedi Effect®) on a new proprietary compound composed of herbs and minerals (herbomineral formulation). The test formulation contains the mixture of the herbal root extract ashwagandha and the minerals (zinc, magnesium, and selenium). The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated formulation. The immunomodulatory parameters viz. humoral immune response (IgG and IgM), cellular immune response (CD4+/CD8+), hematological parameters, lipid profile, hepatic enzymes, and sex hormones (progesterone and estrogen) in female Sprague Dawley rats were analyzed. The results of humoral immune response data showed decreased levels of IgG and IgM by 80.54% and 78.94% respectively, in the Biofield Energy Treated test formulation group (G3) compared with the disease control group (G2). Estimation of cellular immune response data revealed a significant (p≤0.01) increase in the ratio of CD4+/CD8+ by 161.71% in the G3 group, while it was increased by 100.50% in the untreated test formulation group (G4) compared with the G2 group. Thus, the humoral and cellular immune responses were significantly altered in the G3 as compared with the G4 group. The TLC and neutrophils counts were significantly increased by 11.34% and 1.34% respectively, in the G3 compared with the G2 group. Lipid profile data suggested that the Biofield Energy Treated test formulation showed improvement in the tested parameters compared with the untreated test formulation group. However, significant improvement was found in the tested hepatic biomarkers i.e. decreased levels of the SGOT, SGPT, and CK-MB by 14.28%, 8.54%, and 20.79% in the G3 group compared with the G2 group. The levels of total protein, alkaline phosphatase, albumin, and globulin were increased by 11.93%, 1.24%, 6.10%, and 20.74% respectively, in the G3 compared with the G2 group. Consequently, the levels of progesterone and estrogen were significantly increased by 199.86% and 50.19%, respectively in G3 with respect to the G2 group. In conclusion, overall data suggest that The Trivedi Effect®-Biofield Energy Healing Treatment on the herbomineral formulation can be used for autoimmune and inflammatory diseases, stress management, and anti-aging by improving overall health.
Abstract: The aim of the present study was to evaluate the immunomodulatory potential of Biofield Energy Treatment (The Trivedi Effect®) on a new proprietary compound composed of herbs and minerals (herbomineral formulation). The test formulation contains the mixture of the herbal root extract ashwagandha and the minerals (zinc, magnesium, and selenium). The...
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Construction and Functional Analysis of Luciferase Reporter Plasmid Containing CD25 Gene Promoter
Yuan Xiang,
Mei Wu,
Jia-Peng Li,
Zi-Jian Zhang,
Zhou-Tong Dai,
Feng Huang,
Han-Han Li,
Xing-Hua Liao
Issue:
Volume 5, Issue 6, December 2017
Pages:
160-163
Received:
27 December 2017
Published:
28 December 2017
Abstract: CD25 is the alpha chain of IL-2R and IL-2R is distributed on the surface of activated T cells, B cells, and NK cell. The transcriptional factor CD25 plays an important role in the proliferation and differentiation of Treg cells. MKL-1 is also called MAL (megakaryocytic acute leukemia) and belongs to the Myocardin family which also includes MKL-2 (megakaryoblastic leukemia 2) and Myocardin. Studies have shown that in the process of mouse megakaryocyte differentiation, the expression of MKL-1 is up-regulated, and MKL-1 promotes the physiological maturation of human and mouse megakaryocytes. However, the exact mechanism of the CD25 on megakaryocytes is unclear. In this study, human CD25 promoter luciferase reporter construct was generated by PCR amplification. Then the PCR fragment was digested and cloned into pGL3 vector, and this promoter sequence was verified by sequencing. The result showed that luciferase reporter with CD25 promoter was successfully constructed. The expression vectors of human MKL-1 were bought from addgene. Then the activation of the CD25 promoter was detected in 293T cells by luciferase reporter assay after transfected expression vectors of human MKL-1. The result showed that transfected the expression vectors can enhance the transcriptional activity of CD25. Our research will reveal the effect of CD25 on megakaryocytes and may provide a theoretical basis and therapeutic method for diseases caused by megakaryocytes.
Abstract: CD25 is the alpha chain of IL-2R and IL-2R is distributed on the surface of activated T cells, B cells, and NK cell. The transcriptional factor CD25 plays an important role in the proliferation and differentiation of Treg cells. MKL-1 is also called MAL (megakaryocytic acute leukemia) and belongs to the Myocardin family which also includes MKL-2 (m...
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Incorporating Novel Ecosystems and Layered Landscapes for Ecological Restoration in Cities
Issue:
Volume 5, Issue 6, December 2017
Pages:
164-169
Received:
1 December 2017
Accepted:
10 December 2017
Published:
2 January 2018
Abstract: Ecological restoration in cities is problematic. The natural environment is radically altered with large areas of impervious surfaces, invasive species and a parallel artificial infrastructure for transporting water, waste and energy. There are also competing goals to meet the needs of people as identified in Official Community Plans. Cities are novel ecosystems and the restoration targets often do not align well with the natural ecosystems that historically existed in the area. The objective of this study is to use the University of Victoria campus as an example of urban restoration that incorporates novel ecosystems and takes a layered landscapes approach to address the complex socio-ecological histories of a site. Hobbs Creek and its ravine, Mystic Vale, are a particular focus. Instead of trying to restore the original fish-bearing stream and forest, the University of Victoria has used a novel ecosystem approach to set a suitable restoration target for an ecosystem that is capable of maturing, has a stable trajectory and is resilient. The university has an ongoing program of invasive species removal, reducing stream bank erosion and managing human impacts that is consistent with applying the layered landscapes concept, addressing the diverse values and visions of people as well as ecological integrity. In cities, the novel ecosystem approach acknowledges that complex socio-ecological histories of a site have shaped them over time and play a major role in determining a site’s future. Combining the layered landscapes perspective with the novel ecosystems concept as was done in this project is an effective approach to ecological restoration in cities.
Abstract: Ecological restoration in cities is problematic. The natural environment is radically altered with large areas of impervious surfaces, invasive species and a parallel artificial infrastructure for transporting water, waste and energy. There are also competing goals to meet the needs of people as identified in Official Community Plans. Cities are no...
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