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Volume 3, Issue 6-1, November 2015, Page: 45-52
Greenwater, Marine Bacillus subtilis HS1 Probiotic and Synbiotic Enriched Artemia and Rotifers Improved European Seabass Dicentrarchus labrax Larvae Early Weaning Length Growth, Survival, Water and Bacteriology quality
Salem Ahmed Md., Fish Reproduction & Spawning Lab., Aquaculture Division, National Institute of Oceanography & Fisheries (NIOF), Quiet Bay Castle, El-Anfoshy, Alexandria, Egypt
Nour A. M., Animal and Fish Production Dep., Faculty of Agriculture (El-Shatby), Alexandria University
Srour T. M., Animal and Fish Production Dep., Faculty of Agriculture (Saba Basha), Alexandria University
Assem S. S., Fish Reproduction & Spawning Lab., Aquaculture Division, National Institute of Oceanography & Fisheries (NIOF), Quiet Bay Castle, El-Anfoshy, Alexandria, Egypt
Ibrahim H. A., Marine Microbiology Lab., Marine Environment Division, (NIOF), Alexandria, Egypt
El-Sayed H. S., Fish Reproduction & Spawning Lab., Aquaculture Division, National Institute of Oceanography & Fisheries (NIOF), Quiet Bay Castle, El-Anfoshy, Alexandria, Egypt
Received: Sep. 16, 2015;       Accepted: Sep. 16, 2015;       Published: Nov. 29, 2015
DOI: 10.11648/j.ajls.s.2015030601.17      View  3872      Downloads  82
Abstract
The present study conducted in Fish Reproduction & Spawning Lab., NIOF, Alexandria, Egypt. European sea bass Dicentrarchus labrax newly hatched larvae from 8 dph to the 40 dph was reared according to (Süzer et al., 2011). The 6 tanks was green water with N. salina Algal count not less than 300000 cell/ml. The experimental treatments using green water using N. salina as positive control (G), green water plus marine probiotic bacteria (G+MP) and green water plus synbiotic (G+S).4 hours enriched rotifers Brachionus plicatilis started from the 7th dph until the 14th dph, the beginning of cofeeding on 6 hours enriched Artemia fransiscana (GSL) nauplii started and rotifers reduction started in the 18th dph and stopped from the 20th dph and artemia napulii fed alone until the 25th dph, 25dph larvae started feeding on artemia metanauplii and cofeeding with Orange® P 1/2 Small microdiets with 100-200 micron to 35dph. From the 35th dph artemia metanapulii stopped and larvae fed only on O.range® until the end of the experiment (the 40th dph). The treatments were green water using Nanochlorapsis salina algae (G), greenwater plus marine Bacillus subtilis HS1 Probiotic bacteria (G+MP) and greenwater plus synbiotic (G+S) in 30 l tanks in duplicates. Microbiological measurements were performed in water samples in every larvae critical stage (7, 14, 21, 25, 35 and 40dph) for colony forming unit (CFU) of total bacterial count, Vibrio, Staphylococus, Aeromonas and Bacillus. Also water quality measurements were performed in the beginning of the experiment in 2 pm and in the same time in the 7, 14, 21, 28, 35 and 40 dph newly hatched larvae tanks. Finally, the results of the 40dph early weaned larvae showed significantly (p<0.05) higher final total length achieved by (G+MP) followed by (G+S) and the lower significant (p<0.05) recorded by (G) treatment. The bacterial counts of the Aeromonas not detected in all treatments, the other bacterial counts showed promising results of (G+MP) and (G+S) than (G) treatments in both inhibiting potentially pathogenic bacteria counts and also in improving the potentially useful bacterial counts.
Keywords
Marine Probiotic, Synbiotic, Larvae, Early Weaning
To cite this article
Salem Ahmed Md., Nour A. M., Srour T. M., Assem S. S., Ibrahim H. A., El-Sayed H. S., Greenwater, Marine Bacillus subtilis HS1 Probiotic and Synbiotic Enriched Artemia and Rotifers Improved European Seabass Dicentrarchus labrax Larvae Early Weaning Length Growth, Survival, Water and Bacteriology quality, American Journal of Life Sciences. Special Issue: New Horizons in Basic and Applied Zoological Research. Vol. 3, No. 6-1, 2015, pp. 45-52. doi: 10.11648/j.ajls.s.2015030601.17
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