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Volume 2, Issue 6-2, December 2014, Page: 19-25
Management Fusarium Wilt of Sweet Pepper by Bacillus Strains
Abada K. A., Plant Pathol. Dept., Fac. Agric.,Cairo Univ. Giza, Egypt
M. A. Ahmed, Plant Pathol. Dept., Fac. Agric.,Cairo Univ. Giza, Egypt
Received: Nov. 7, 2014;       Accepted: Nov. 12, 2014;       Published: Nov. 25, 2014
DOI: 10.11648/j.ajls.s.2014020602.13      View  3744      Downloads  378
Isolation trials from the roots of wilted sweet pepper plants yielded Alternaria spp., Fusarium oxysporum, Pythium spp., Rhizontonia solani , Sclerotium rolfsii and Trichoderma spp. The isolates of the fungus F. oxysporum were selected to test their pathogenicity and Kalubia isolate was the most virulent one. The fungus was virulent to sweet pepper and low infection was found in case of the other five tested plants. Therefore, the fungus F. oxysporum named Fusarium oxysporum f.sp. capsici. All the five Bacillus strains, i.e. Bacillus chitinosporus ,B. coagulans , B. humilus ,B.subtilis and B. thuringiensis caused significant reduction to the radial growth of F.o.f.sp.capsici compared with control treatment. In addition, the growth of the tested pathogen was completely inhibited by B.subtilis and B.thuringiensis at the concentration of 60%. Furthermore, B.thuringiensis was the most efficient bioagent in this regard followed by B.subtilis then B.pumilus. Adding the three tested bioagents ,i.e.B.pumilus .B.subtilis and B. Thuringiensis to soil infested with F.o.f.sp.capsici resulted in significant reduction to sweet pepper wilt with significant increase to the plant height as well as the number of pods and their weight / plant compared with control treatment. The symptoms of the disease were obvious both on the foliage growth and the xylem vesicles, but the severity of the disease was more higher on the xylem vesicles than on the foliage growth. In addition, plants grown in soil infested with Bacillus strains were of high values of plant height and fruit yield (number and weight / plant) than that grown in the control (uninfested soil). The total phenolic compounds were greatly increased in the bacterial treated plants as compared to untreated plants with the bioagents and that infested with the pathogen only. These results give a potential of these bacterial strains for use as plant protection agents against Fusarium wilt of sweet pepper. This work was performed to investigate the potential of some bacterial bioagents ,i.e. B. humilus , B.subtilis and B. thuringiensis on management of sweet pepper Fusarium wilt and the formation of phenolic compounds in the plants.
Bacillus spp,Bioagents, FusariumWilt, Sweet Pepper, Total Phenolic Compounds
To cite this article
Abada K. A., M. A. Ahmed, Management Fusarium Wilt of Sweet Pepper by Bacillus Strains, American Journal of Life Sciences. Special Issue: Role of Combination Between Bioagents and Solarization on Management of Crown-and Stem-Rot of Egyptian Clover. Vol. 2, No. 6-2, 2014, pp. 19-25. doi: 10.11648/j.ajls.s.2014020602.13
Abada K.A.(1994). Fungi associated with root-rot of pepper and some factors affecting disease incidence. 7 th Cong. of Phytopathol., April, Cairo, Egypt,:219-226 .
Abada K.A. and Eid Kh. E. ( 2014). A Protocol suggested for management of canta-loupe downy mildew . American Journal of Life Sciences, 2(3):1-10.
Attia M.F. and Abada K.A. (1994) . Control of wilt and root-rot of pepper. 7th Cong. of Phytopathol., April, Cairo, Egypt: 397-409 .
Akram ,W.; Mahboob A. and Javel A.A.(2013). Bacillus thuringiensis strain 199 can induce systemic resistance in tomato against Fusarium wilt .Europ.J.of Mirobiol. and Immunol., 275-280.
Amini J. and Sidovich, D. F. (2010). The effects of fungicides on Fusarium oxysporum f. sp. lycopersici associated with Fusarium wilt of tomato. J. of Plant Protec.Res., 50 (2), 175-180.
Albersheim P. and Valent B.S.(1978). Host-pathogen interactions in plants. Plants, when exposed to oligosaccharides of fungal origin, defend themselves by accumulating antibiotics. J. Cell Biol., 78(3):627–643.
Albersheim P. and Anderson-Prouty A.J.(1975). Carbohydrates, proteins, cell surfaces, and the biochemistry of pathogenesis. Ann. Rev. Plant Physiol., 26: 31–52.
Baker C.J.; Orlandi E.W. and Mock N.M.(1993). Harpin, an elicitor of the hypersensitive response in tobacco caused by Erwinia amylovora, elicits active oxygen production in suspension cells. Plant Physiol.,102(4):1341–1344.
BlackL.; Green, S.; Hartman, G. and Poulos J. (1991). Pepper Diseases: A Field Guide. Publication No. 91-347. Asian Vegetable Research and Development Center. P.O. Box 205, Taipei 10099. 98pp.
Brewer M.T. and Larkin R.P. (2005). Efficacy of several potential biocontrol organisms against Rhizoctonia solani on potato. Crop Protection, 24: 939-950.
Booth C.(1971). The genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, England.
De Vleesschauwer D. and Hofte M.. (2009). Rhizobacteria-induced systemic resistance. Adv. Bot. Res.,;51:223–281.
Domsch K.H.; Gams W. and Anderson T.H. (1980). Compendium of Soil Fungi. Vol. 1 and 2, Academic press. London.
Fisher R.A., 1948. Statistical Methods 6th ed. Iowa State Univ. Press, Ames, Iowa, USA.
Hammond-Kosack K.E. and Jones J.D.(1996). Resistance gene-dependent plant defense responses. Plant Cell,8 (10): 1773–1791.
He S.Y.; Huang H.C. and Collmer A.(1993 ). Pseudomonas syringae pv. syringae harpin Pss: a protein that is secreted via the Hrp pathway and elicits the hypersensitive response in plants. Cell.,73(7):1255–1266.
Holt J.G. and Krieg N.R(1984). Bergey’s Manual of Systematic Bacteriology. Williams & Wilkins, Baltimore, USA.
Jacobsen B. J., Zidack, N. K. and Larson B. J. 2004. The role of Bacillus-based biological control agents in integrated pest management systems: Plant diseases. Phytopathology,94:1272-1275.
Kloepper J.W.; Ryu C.M. Zhang S.(2004). Induced systemic resistance and promotion of plant growth by Bacillus spp. Phytopathology,94(11):1259–1266.
Oedjijono M. A. L. and Dragar C. (1993). Isolation of bacteria antagonistic to a range of plant pathogenic fungi. Soil Biol. Biochem., 25: 247–250.
Parry J.M.; P.C.B. Turnbull and J.R. Gibson (1983). A colour atlas of Bacillus species, Wolfe Medical Publications Ltd.
Snedecor G.W. and Cochran W.G. 1967. Statistical Methods. 6th Ed. Iowa State. Univ. Press, Ames, Iowa, USA.
Szczech M. and Shoda M. (2006).The effect of mode of application of Bacillus subtilis RB14-C on its efficacy as a biocontrol agent against Rhizoctonia solani. J Phytopathol.,154:370–377.
Ulloa M..; Hutmacher R . B.; Davis R . M..; Wright S. D.; Percy R., and Marsh B. (2006).Breeding for Fusarium wilt race 4 resistance in cotton under field and greenhouse conditions. The J. of Cotton Sci., 10:114–127.
van Loon L.C.; Bakker P.A. and Pieterse C.M..(1998). Systemic resistance induced by rhizosphere bacteria. Ann. Rev. Phytopathol., 36:453–483.
Van Loon L.C. (2007).Plant responses to plant growth-promoting rhizobacteria. Eur. J. Plant Pathol., 119:243–254.
Van Peer R.; Niemann G.N. and Schippers B.(1991). Induced resistance and phytoalexin accumulation in biological control of Fusarium wilt in carnation by Pseudomonas sp. strain WCS417r. Phytopathology,81:728–734.
Veit S.; Wörle J.M.; Nürnberger T.; Koch W. and Seitz H.U.(2001). A novel protein elicitor (PaNie) from Pythium aphanidermatum induces multiple defense responses in carrot, Arabidopsis, and tobacco. Plant Physiol.,127(3):832–841.
Waterman P.G. and Mole S. (1994).Analysis of Phenolic Plant Metabolites. London: Blackwell Sci. Publ., Method in Ecology.
Xing L.; Ding Z.; Wenxiang Y.;. Li D and Daqun L. (2003). A study on the effect ofBacillus on downy mildew of cucumber. Plant Protection, 29(4): 25-27.
Yu L.M.(1995). Elicitins from Phytophthora and basic resistance in tobacco. Proc. Natl. Acad. Sci. U S A., 92(10):4088–4094.
Yu X.; Ai C. ; Xin L.and Zhou G.(2011).The siderophore-producing bacterium, Bacillus subtilis CAS15, has a biocontrol effect on Fusarium wilt and promotes the growth of pepper. Eur. J. Soil Biol.,47(2):138-145.
Zaher,Effat A., Abada , K.A. and Zyton,Marwa A. (2013).Effect of combination between bioagents and solarization on management of crown-and stem-rot of Egyptian clover. J.of Plant Sci.,1 ( 3):43 -50 .
Zieslin N. and Ben-Zaken R.(1993). Peroxidase activity and presence of phenolic substances in peduncles of rose flowers. Plant Physiol. Biochem.,31:333–339.
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