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Volume 2, Issue 6, December 2014, Page: 382-388
Isolation and Characterization of Lipid-Degrading Bacteria in Wastewater of Food Processing Plants and Restaurants in Can Tho City, Vietnam
Ngo Thanh Phong, Dept. of Biology, College of Natural Sciences, Can Tho University, Can Tho City, Vietnam
Nguyen Thanh Duyen, Center for Continuing Education of Cairang district, Can Tho city, Vietnam
Cao Ngoc Diep, Dept. Microbiology Biotechnology, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam
Received: Nov. 25, 2014;       Accepted: Dec. 5, 2014;       Published: Dec. 31, 2014
DOI: 10.11648/j.ajls.20140206.18      View  3659      Downloads  412
High lipid (fats and oils) concentration contained in wastewater inhibits the activity of microbes in biological wastewater treatment systems. The lipids degradation capability of lipid-degrading bacteria was investigated for possible application in treatment of lipids-contaminated wastewater. One hundred and two bacterial isolates were isolated from 43 vegetable oil- contaminated wastewater samples of many food processing plants and restaurants in 5 districts of Can Tho city, Vietnam on LB medium. There were sixty-one isolates produced clear zones on Tw20 medium, only eleven of which were found to have the high ability to degrade vegetable oil in the contaminated wastewater. These eleven isolates were identified by PCR technique and DNA sequencing. The results of DNA sequencing were compared with GenBank database of NCBI by BLAT N software. The sequences from selected isolates showed high degrees of similarity to those of the GenBank references (between 97% and 99%). Two isolates belonged to Bacilli (18.18%) and nine isolates belonged to Gammaproteobacteria (81.82%). Based on Pi value (nucleotide diversity), Gammaproteobacteria group had the highest Theta values. Theta value (per sequence) from S of SNP for DNA polymorphism were calculated for each group and 11 strains of lipid-degrading bacteria had high genetic diversity. The results propose Acinetobacteria soli strain AL3 a potential bioproduct for wastewater treatment because of its high ability of lipid degradation and biosafety.
Acinetobacteria, Bacilli, Food Processing Plants and Restaurants, Lipid Contaminated Wastewater, Lipid Degradation, Vegetable Oil
To cite this article
Ngo Thanh Phong, Nguyen Thanh Duyen, Cao Ngoc Diep, Isolation and Characterization of Lipid-Degrading Bacteria in Wastewater of Food Processing Plants and Restaurants in Can Tho City, Vietnam, American Journal of Life Sciences. Vol. 2, No. 6, 2014, pp. 382-388. doi: 10.11648/j.ajls.20140206.18
V. Cipinyte, S. Grigiskis and E. Baskys, “Selection of fat-degrading microorganisms for the treatment of lipid-contaminated environment,” Biologija, , vol. 35, pp. 84-92, 2009.
M. Yada, K. Kawakuchi, and Y. Mihara, “Haikibutsu no bioconversion,” Bioconversion of wastes, Chijin Shokan, Tokyo, 2001.
M.C. Cammarota, G.A. Teixeira, and D.M.G. Freire, “Enzymatic pre-hydrolysis and anaerobic degradation of wastewaters with high fat contents,” BioTechnol. Letters, vol.23, pp.1591-1595, 2001.
D. Sugimori, and T. Utsue, “A study of the efficiency of edible oils degraded in alkaline conditions by Pseudomonas aeruginosa SS-219 and Acinetobacter sp. SS-192 bacteria isolated from Japanese soil,” World J. Microbiol. Biotechnol., vol. 28(3), pp.841-848, 2013. doi:10.1007/s11274-011-0880-6.
M. Hatamoto, H. Imachi, Y. Yashiro, A. Ohashi and H. Harada, “Diversity of Anaerobic Microorganisms Involved in Long-Chain Fatty Acid Degradation in Methanogenic Sludges as Revealved by RNA-Based Stable isotope Probing,” Appl. And Environ. Microbiology, vol. 73(13), pp.4119-4127, 2007.
D. Sugimori, M. Nakamura, and M. Mihara, “Microbial degradation of lipid by Acinetobacter sp. strain SOD-1,” Biosci. Biotechnol. Biochem, vol.66(7), pp.1579-1582, 2002.
D. Sugimori, M. Watanabe and T. Utsue, “Isolation and lipid degradation profile of Raoultella planticola strain 232-2 capable of efficiently catabolozing edible oils under acidic conditions,” Appl. Microbiol. Biotechnol., vol. 97, pp;871-880, 2013.
J.P. Canler, C. Roger and Ph. Duchene, “Aerobic biological treatment of grease from urban wastewater treatment plants,” Water Sci. Technol., vol. 44, pp.219-226, 2001.
Y. Wei, R.T.V. Houten, A.R. Borger, D.H. Eikelboom, and Y. Fan, “Minimization of excess sludge production for biological wastewater treatment,” Water Res., vol. 37, pp.4453-4467, 2003.
Y. Yamaoka, K. Takeno, H. Shinkawa, N. Noparatnaraporn and K. Sasaki, “Isolation of a thermotolerant photosynthetic bacterium, Rhodobacter spaeroides strain, NAT, and its capasity for oil and chemical oxygen demand removal at high temperatures,” Biosci. Biotechnol. Biochem., vol. 72(6), pp.1601-1603, 2008.
D, Tanaka, M. Takashima, M. Mizuta, S Tanaka, A. Sakatoku, A. Nishikawa, T. Osawa, M. Noguchi, S.I. aizawa and S. Nakamura, “Acinetobacter sp. Ud-4 efficiently degrades both edible and mineral oils: Isolation and charactization,” Curr. Microbiol, vol. 60(3), pp.203-209, 2010.
N.F. Gray, “Biology of wastewater treatment,” Oxford University press, pp.6-38, 1989.
S Bennasar A., C. Guasp and J. Lalucat, “Molecular methods for the detection and identification of Pseudomonas stutzeri in pure culture and environmental samples,” Microbiol. Ecol., vol.35, pp:22-33, 1998.
Y. Matsumiya, D. Wakita, A. Kimura, S. Sanpa, and M. Kubo, “Isolation and characterization of a lipid-degrading bacterium and its application to lipid-contaning wastewater treatment,” J. Biosci. Bioeng., vol 103(4), pp. 325-330, 2007.
E. El-Bestawy, M. H. El-Masry and N. El-Adl, “The potentiality of free Gram negative bacteria for removing oil and grease from containing industrial effluents,” World Journal of Microbiology and Biotechnology, vol. 21, pp:815-822, 2005.
B. Neumann, A. Pospiech, and H.U. Schairrer, “Rapid isolation of genomic DNA from Gram-negative,” Trends Gent., vol. 8, pp. 332-333, 1992.
D.J. Lane, “16S/23S rRNA sequencing,” Nucleic acid techniques in bacterial systematics, pp. 115-175. Edited by E. Stackebrandt and M.Goodfellow. NewYork: John Wiley, 1991.
S. Turner, K.M. Pryer, V.P.W. Miao and J.D. Palmer, “Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis,” Journal of Eukaryotic Microbiology, vol.46, pp:327-338, 1999.
K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar, “MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likehood, Evolutionary Distance and Maximum Parsimony Methods,” Mol. Biol. Evol., vol. 28, pp. 2731-2739, 2011.
M.K. Halushka, J.B. Fan, K. Bentley, L. Hsie, N. Shen, A. Weder, R. Cooper, R. Lipshutz, and A. Charavarti, “Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homestasis,” Nat. Genet., vol. 22(3), pp. 239-247, 1999.
D. Paparaskeas, P. Christakopoulas, D. Kekos, and J.B. Marcis, “Optimization production of extracellular lipase from Rhodorula glutinis,” Biotechnology Letters, vol. 1, pp. 397-402, 1992.
M. Domenico, A. Guidice, L. Michaud, Pollar Res., vol. 23(2), pp.141-161, 2004.
G.A. Watterson, “On the number of segregation sites in general models without recobination,” Theor. Pop. Biol., vol. 7, pp. 256-276, 1975.
J. Rozas, and R. Rozas, “DnaSP version 4.1: an integrated program for molecular population genetics and molecular evolution analysis,” Bioinformatics, vol. 15, pp. 174-175, 2005.
C. Ruiz, F.I.J. Pastor and P. Diaz, “Isolation of lipid- and polysaccharide-degrading microorganisms from subtropical forest soil, and analysis of lipolytic strain Bacillus sp. CR-179,” Letters in Applied Microbiology, vol. 40, pp:218-227, 2005.
S. Cappello, S. Santisi, R. Calogero, M. Hassanshahian, M.M. Yakimov, “Charactisation of Oil-Degrading Bacteria Isolated from Bilge Water,” Water Air Soil Pollut., vol. 223, pp:3219-3226, 2012.
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