American Journal of Life Sciences

Volume 2, Issue 4, August 2014

  • Standarizing in Planta Agrobacterium Tumefacience Mediated Genetic Transformation Protocol to Develop New Events by Transforming G. Hirsutum Cotton based on Cry1Ac-Cry1Ec Genes

    Yanal A. Alkuddsi, Shreekanth S. Patil, S. M. Manjula, K. J. Pranesh, B. C. Patil

    Issue: Volume 2, Issue 4, August 2014
    Pages: 190-199
    Received: Jul. 13, 2014
    Accepted: Jul. 22, 2014
    Published: Aug. 10, 2014
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    Abstract: Cotton breeding for insect resistance has been limited by a lack of sufficient genetic variation in the existing germplasms. Therefore, genetic engineering provides the possibility of creating varieties carrying new properties coming even from heterologous source. Exogenous pesticidal transgenes can be introduced into plants. Agrobacterium mediated... Show More
  • Asymptomatic Carriage of Plasmid-Mediated Multidrug-Resistant Staphylococcus aureus in Nasal Tracts of Persons in a Semi-Urban Area of Nigeria

    Jonathan Osariemen Isibor, Ehima Otabor

    Issue: Volume 2, Issue 4, August 2014
    Pages: 200-204
    Received: Jul. 22, 2014
    Accepted: Jul. 30, 2014
    Published: Aug. 10, 2014
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    Abstract: Staphylococcus aureus remains one of the most important opportunistic bacterial pathogens of man and strains resistant to available drug classes have become more and more prevalent in clinical and community settings. The aim of this study was to determine the nasal carriage rates of multidrug – resistant (MDR) Staph aureus in food vendors and hospi... Show More
  • Protective Effect of Aspirin on γ Radiation-Induced Sperm Malformations in Swiss Albino Male Mice

    Marowa Mansour AlMathkour, Entissar Suliman AlSuhaibani

    Issue: Volume 2, Issue 4, August 2014
    Pages: 205-210
    Received: Aug. 04, 2014
    Accepted: Aug. 11, 2014
    Published: Aug. 20, 2014
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    Abstract: This study evaluated the effects of γ radiation on spermatozoa based on the morphological characteristics of sperm in the caudal epididymis of SWR/J mice. In this study, various abnormal sperm shapes (amorphous heads, hook-less, mid-piece defect, pinheads, coiled tails, and tail-less) were observed after exposure to γirradiation (2 or 4 Gy)and afte... Show More
  • Histopathological Alterations in the Gill, Liver and Brain of Cyprinus Carpio on Exposure to Quinalphos

    Ramesh Raju Chamarthi, Manjunatha Bangeppagari, Jaffer Mohiddin Gooty, Srinivasulu Mandala, Juan Ortiz Tirado, Shambanagouda R. Marigoudar

    Issue: Volume 2, Issue 4, August 2014
    Pages: 211-216
    Received: Mar. 18, 2014
    Accepted: May 20, 2014
    Published: Aug. 30, 2014
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    Abstract: Study was conducted to assess the histopathological damage of Gill, Liver and Brain in common carp, Cyprinus carpio after sublethal exposure to Quinalphos. Exposed to sublethal concentration (One tenth (1/10th, 0.75 μl/L) of commercial grade quinalphos (25% Emulsified Concentration) for 1, 7, 14, 21 and 30 days and a parallel control was run simult... Show More
  • Expression of O6 Methyl Guanine Methyl Transferase (Mgmt) in Oral Squamous Cell Carcinoma Patients in Alexandria, Egypt (Smokers and Non-Smokers)

    Hend Mohamed Helmy, Taissir Ali Omar, Sahar Mohamed ElSheikh, Ahmed Serag Habib

    Issue: Volume 2, Issue 4, August 2014
    Pages: 217-223
    Received: Aug. 06, 2014
    Accepted: Aug. 16, 2014
    Published: Aug. 30, 2014
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    Abstract: Objectives: O6-methylguanine-DNA methyltransferase (MGMT) removes mutagenic, carcinogenic, and cytotoxic adducts from O6-methylguanine in DNA through a direct reversal mechanism. Decreased expression of MGMT has been reported in a variety of human malignant tumors. The purpose of this study was to clarify the correlation of MGMT expression levels i... Show More
  • Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea Mays L.) Cultivated on Acrisols of the Southeast of Vietnam

    Dang Thi Ngoc Thanh, Cao Ngoc Diep

    Issue: Volume 2, Issue 4, August 2014
    Pages: 224-233
    Received: Aug. 11, 2014
    Accepted: Aug. 19, 2014
    Published: Aug. 30, 2014
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    Abstract: Endophytic bacterial diversity in Maize plant cultivated on Acrisols of the Eastern of South Vietnam was studied. Maize material was collected from five sites (provinces/cities) of this region. Endophytic bacteria were isolated in two kinds of medium (LGI, NFb) together with 16S rRNA gene fragments amplified from DNA using eubacterial universal pri... Show More
  • TNF-α Genetic Polymorphisms and its Expression in Egyptian Rheumatoid Arthritis Patients

    Afaf K. Mousa, Ahmed M. Ghoneim, Noha B. El-Mashad, Ahmed E. El-Ghobashy

    Issue: Volume 2, Issue 4, August 2014
    Pages: 234-240
    Received: Aug. 15, 2014
    Accepted: Aug. 23, 2014
    Published: Aug. 30, 2014
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    Abstract: Rheumatoid arthritis (RA) is a complex, multifactorial, inflammatory disease that affects more than 1.5 million adults. The current study aimed to investigate whether there is an association between either -376 G/A or -238 G/A polymorphisms of TNF-α promoter and the risk of RA in Egyptian patients, and investigate whether these polymorphisms are li... Show More
  • Ramipril and Valsartan as Protective Agents against Some Complications of an Experimentally Induced Obesity in Rats

    Said Al-Dalaen, Aiman Al-Qtaitat, Samir Mahgoub, Nedal Alnawaiseh, Jehad Al-Shuneigat, Samih Sarayreh, Yousef Sarayreh

    Issue: Volume 2, Issue 4, August 2014
    Pages: 241-250
    Received: Aug. 03, 2014
    Accepted: Aug. 27, 2014
    Published: Sep. 10, 2014
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    Abstract: Objectives: Our work aimed to study the effect of experimentally induced obesity on the rate of advanced glycated end products (AGEs) formation and the activity of angiotensin converting enzyme (ACE) and its relation to oxidative stress and also to evaluate the protective effect of ramipril (an angiotensin converting enzyme inhibitor), valsartan [a... Show More